Journal: PLoS ONE
Article Title: Motor Unit Abnormalities in Dystonia musculorum Mice
doi: 10.1371/journal.pone.0021093
Figure Lengend Snippet: A . Alpha-internexin staining (green) in cell bodies within the DRG of wild type mice. RT-97 staining of phosphorylated neurofilaments (red) is limited to a few cells. B . The DRG from dt 27J mice is smaller and has fewer ganglion cells per DRG compared to wild type DRGs. RT-97 staining of phosphorylated neurofilaments (red) can be readily seen within the perikarya of many ganglion cells while alpha-internexin staining (green) is also present in a few cells. Scale bar, 10 µm (A, B). C . Ventral horn region of the L1 spinal cord from a wild type mouse showing no accumulation of phosphorylated neurofilaments within the perikarya of motor neurons (white arrows). Alpha-internexin staining (green) is observed throughout the dendrites and axons of the motor neurons. D . Ventral horn region of the L1 spinal cord from a dt 27J mouse showing abnormal accumulation of phosphorylated neurofilaments within the perikarya (white arrows) of motor neurons. In comparison, alpha-internexin staining (green) is observed in dendrites and axons. Scale bar, 20 µm (C, D). E – G . TUNEL assay showed no labeling in cells in the L1 spinal cords of WT and dt 27J mice (F–G). DNase treated L1 spinal cords were used as a positive control (E). Scale bar 10 µm.
Article Snippet: Mousemonoclonal anti-neurofilament RT-97 specific for phosphorylated NF-H , Wistar ratneurofilaments , Developmental StudiesHybridoma Bank (RT-97) , 1∶3 (I) , It recognizes a band at 200 KDa corresponding to NF-H protein (manufacturer's technical information)..
Techniques: Staining, TUNEL Assay, Labeling, Positive Control